Review



complete egm ec culture medium  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Lonza complete egm ec culture medium
    ( a ) Phosphorylation of Smad1/5 and Smad2/3 <t>in</t> <t>HUVECs</t> undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete <t>EGM-2</t> medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots
    Complete Egm Ec Culture Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+egm+ec+culture+medium/pmc05636968-144-8-12?v=Lonza
    Average 90 stars, based on 1 article reviews
    complete egm ec culture medium - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "The functional relationship between transglutaminase 2 and transforming growth factor β 1 in the regulation of angiogenesis and endothelial–mesenchymal transition"

    Article Title: The functional relationship between transglutaminase 2 and transforming growth factor β 1 in the regulation of angiogenesis and endothelial–mesenchymal transition

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.399

    ( a ) Phosphorylation of Smad1/5 and Smad2/3 in HUVECs undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete EGM-2 medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots
    Figure Legend Snippet: ( a ) Phosphorylation of Smad1/5 and Smad2/3 in HUVECs undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete EGM-2 medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots

    Techniques Used: Phospho-proteomics, Western Blot, Control, Isolation, Cell Recovery, Plasmid Preparation



    Similar Products

    complete egm ec culture medium  (Lonza)
    90
    Lonza complete egm ec culture medium
    ( a ) Phosphorylation of Smad1/5 and Smad2/3 <t>in</t> <t>HUVECs</t> undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete <t>EGM-2</t> medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots
    Complete Egm Ec Culture Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+egm+ec+culture+medium/pmc05636968-144-8-12?v=Lonza
    Average 90 stars, based on 1 article reviews
    complete egm ec culture medium - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Phosphorylation of Smad1/5 and Smad2/3 in HUVECs undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete EGM-2 medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots

    Journal: Cell Death & Disease

    Article Title: The functional relationship between transglutaminase 2 and transforming growth factor β 1 in the regulation of angiogenesis and endothelial–mesenchymal transition

    doi: 10.1038/cddis.2017.399

    Figure Lengend Snippet: ( a ) Phosphorylation of Smad1/5 and Smad2/3 in HUVECs undergoing differentiation into tubular-like structures. Western blot analysis of extracts from the co-cultures was performed to detect phospho-Smad1/5 (p-Smad1/5) or phospho-Smad2/3 (p-Smad2/3) antibodies. Cells treated with a neutralizing antibody (NeutAb) for TGF β (50 μ g/ml) were considered as the negative controls. Total Smad1 and total Smad2/3 antibodies were used as loading controls and the ratios of the band intensities are shown below. ( b ) Phosphorylation of Smad1/5 in the HUVEC Matrigel model. HUVECs seeded Matrigel in complete EGM-2 medium as described in the ‘Materials and Methods’, for 6 h at 37 °C in 5% CO 2 in the presence of DMSO vehicle control or R294 (100 μ M), after which they were isolated using the BD cell recovery reagent as described in the ‘Materials and Methods’. The phosphorylation of Smad1/5 in HUVECs was studied via western blotting. Total Smad1/5 was used as the loading control and the ratios of the band intensities shown below the blots. ( c ) The Smad signalling in dermal fibroblasts was not affected by TG2 inhibitor R294. Dermal fibroblasts were treated with TG2 inhibitor R294 (100 μ M) or vehicle control (0.01% DMSO) for 48 h, and the presence of the phosphorylated Smad1/5 and 2 was detected via western blotting and the total Smad1/5 and 2/3 were used to normalize the data and the ratios of the band intensities shown below. ( d ) The Smad signalling in mouse ECs. The Smad signalling (p-Smad1/5 and 2/3) in wt ECs treated with DMSO (CNTL) or with 100 μ M R294, TG2−/− Vector control ECs, and the TG2 ab ECs was analysed via western blotting, while the total Smad1/5 and 2/3 were also analysed as described in the ‘Materials and Methods’ with the ratios of the band intensities shown below the blots

    Article Snippet: HUVECs (Lonza, Slough, UK) were cultured in complete EGM EC culture medium (Lonza).

    Techniques: Phospho-proteomics, Western Blot, Control, Isolation, Cell Recovery, Plasmid Preparation